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Related post: elicited a response comparable to that of peptides 39 and 41 in Balb/c mice. Peptides 53 and 72 (233-246) elicited high titer IgG peptide antibodies only. Although the T cell proliferation assay has not yet worked well in H-2 k mice, it is apparent that all these peptides contain T cell epitopes as defined in that assay. Peptides 67, 52 (356-369), and 53 were to varying degrees immunogenic in both H-2 d and H-2* mice. Additional data show that priming with a given peptide in the in vivo assay could result in an anamnestic response to linear epitopes not included on the priming peptide, shortly after the mice were given infectious dengue 4 virus intraperitoneally. 11-47 PHS 6040 (Rbv. 1/84) cpo tu-tii DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER Z01 AI 00531-03 LID PERIOD COVERED October 1, 1989 to September 30, 1990 TITLE OF PROJECT (BO characters or less. Title must tit on one line between the borders.) Functional Analysis of Dengue Nonstructural Proteins NS2B and NS3 PRINCIPAL INVESTIGATOR (bst other professional personnel below Buy Duricef Online the Pnncipal Invesogator ) (Name, title, laboratory, and institute affiliation) PI: Barry Falgout, Ph.D. Others: Michele Pethel Ching-Juh Lai, Ph.D. IRTA Microbiologist Head, MVB Section LID, Duricef Antibiotic NIAID LID, NIAID LID, NIAID COOPERATING UNITS (if any) LAB/BRANCH Laboratory of Infectious Diseases SECTION Molecular Viral Biology Section INSTITUTE AND LOCATION NIAID, NTH, Bethesda, MP 20892 TOTAL MAN- YEARS: 0.6 PROFESSIONAL: 0.4 0.2 CHECK APPROPRIATE BOXfES) □ (a) Human subjects □ (a1) Minors □ (a2) Interviews □ (b) Human tissues Lj3 (c) Neither SUMMARY OF WORK Duricef 500 Mg (Use standard unreduced type. Do not exceed the space provided.) We have employed vaccinia virus as a vector to express various portions of the dengue type 4 virus genome in order to study proteolytic processing in the nonstructural protein (NS) region. This study was carried out in part to test the processing model proposed recently predicting that NS3 is a protease based on limited sequence homology to serine proteases. Indirect evidence showing that NS3 can act in trans to cleaye NS2A/NS2B and NS2B/NS3 was obtained from co-infection experiments using vNS2A-NS2B and vNS2A-ANS2B(10)-30%NS3 or vNS2A-ANS2B(20)-30%NS3 in Buy Duricef which full length NS2B was produced. However, co-infections using Duricef 500 vNS2A-NS2B or vNS2A-NS2B-12%NS3 and vNS3 or v30%NS3 did not demonstrate efficient trans-acting activity of NS3. The reason for this discrepancy is not known. Our results demonstrate that NS2B is also required for these NS cleavages. NS2B acts in trans to facilitate NS2B/NS3 and NS2A/NS2B cleavage. Thus, both NS2B and NS3 together appear to be required for a protease activity that cleaves NS2A/NS2B and NS2B/NS3. In addition, the protease activity presumably is also responsible for the NS3/NS4A and NS4B/NS5 cleavages. Our results also showed that NS2B is needed for the NS3/NS4A cleavage. This activity of NS2B is either ex- acting or very inefficiently trans-acting. 11-48 PHS 6040 (Rev 1/84) SPO «U-»il DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER Z01 AI 00556-02 LID PERIOD COVERED October 1, 1989 to September 30, 1990 TITLE OF PROJECT (80 characters or less. Title must lit on one line between the borders.) Epitopes on Dengue Virus Nonstructural Protein NS1 that Mediate Protection Against Dengue PRINCIPAL INVESTIGATOR (List other professional personnel below the Principal Investigator.) (Name, title, laboratory, and institute affiliation) PI: Hiroyuki Hori, M.D. Others: Ching-Juh Lai, Ph.D. Visiting Associate LID, NIAID Head, MVB Section LID, NIAID COOPERATING UNITS (if any) Rochester General Hospital, University of Rochester, Antibiotic Duricef Rochester, NY (Schlesinger) LAB/BRANCH Laboratory of Infectious Diseases SECTION Respiratory Viruses Section INSTITUTE AND LOCATION NIAID, NTH, Bethesda, MP 20892 TOTAL MAN-YEARS: 1.1 PROFESSIONAL: 1.0 0.1 CHECK APPROPRIATE BOX(ES) □ (a) Human subjects D (a1) Minors □ (a2) Interviews
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